Place it near the end of an anime gag, just after the visual punchline, then follow it with the Anime Ahh sound for a bigger reaction, or pair it before Yamete Kudasai when the joke escalates. Keep it as a single beat inside a fast edit so the next clip can take over.
About this sound
This is a very short anime moan, lasting under a second, built around a single high-pitched vocal sound with no words or music behind it. It feels cheeky, awkward and deliberately over-the-top, the kind of sound that makes a suggestive moment feel playful rather than serious. Its brevity keeps the joke quick and easy to repeat.