Place it as the middle beat of a prank sequence, after a dun dun dun build and before a classic punch sound, then follow with a bone crack for the aftermath.
About this sound
This is a short, roughly five-second impact effect built around a single punch landing on a face covered by a bag, with a dull, muffled thud and the rustle of fabric around it. It feels sudden and awkward, funny because the setup is so over-the-top, yet sharp enough to make a listener wince. It works best as a blunt comic hit rather than a realistic fight sound.