Put it just before a bigger reaction sound in a short edit, letting it set up the gag before the payoff lands. Follow it with Anime Wow for a surprised character moment, or pair it with SpongeBob Fail when a plan falls apart on screen.
About this sound
This is a 1.7-second cartoon mouth sound, a short mouth-made effect in the style of the exaggerated noises used in animated scenes. It feels silly and light-hearted, turning a small on-screen moment into something deliberately over-the-top and fun to hear again.