This sound comes from Cuphead, the run-and-gun boss rush game released by Studio MDHR in 2017. The “Knockout!” voice line plays at the end of successful boss phases and became one of the game’s most recognizable audio cues. It spread through gameplay clips, meme edits and short-form videos because it clearly marks a decisive finish in less than three seconds. Its vintage cartoon presentation helps it stand out, so creators still use it whenever they want a dramatic arcade-style victory cue.