Place it at the start of the reaction beat, then follow the pet's response with Anime Wow for an exaggerated gasp, and save Vine Boom for the moment the joke lands and the edit cuts away.
About this sound
This 10-second clip plays a very high whistle modelled on the tone of a training whistle, a sound many people hear only faintly. It feels abruptly funny and confusing, like a sudden interruption that makes everyone in the room look up at once. It comes from that training tool itself, designed to catch a dog's attention over a distance.