Place it at the end of a reaction sequence in your edit, right after the setup clip finishes. Follow it with a bruh sound button for a deflated beat, or precede it with an emotional damage sound button when the story is building toward an embarrassing reveal.
About this sound
Dr Puñalon is a short meme clip built around that single name as a punchline-style callout. It lands as a playful, slightly absurd reaction, the kind of quick hit that turns an ordinary moment into a joke between friends who already know the reference, without needing any setup or explanation to get the point across instantly.