É claro que não is a Portuguese expression meaning of course not, and this short clip has circulated as a reusable reaction sound rather than as a quotation tied to one widely documented scene. Its appeal is in its brevity and tone: at under two seconds, it can be dropped into a video, meme or chat message without interrupting the joke. Portuguese-speaking creators use it to answer exaggerated claims, false excuses and obvious questions with dry irony, which is why it remains a handy shorthand for disbelief online.