Place it at the end of an escalating sequence, after the reveal and before any outro, so it closes the bit. Pair it with Nope for a refusal first, or follow it with FBI Open Up when the joke needs a bigger escalation after the stop.
About this sound
This is a roughly three-second spoken line, delivered in a flat, weary voice: “It’s time to stop.” It feels like a blunt, deadpan verdict, funny because it sounds exhausted rather than angry, and it works as an instant judgement on something overdone. The line is associated with Filthy Frank, whose meme edits helped spread it in the mid-2010s.