The Markiplier “E” meme spread online through YouTube meme edits, reposts, and short reaction clips built around a distorted image of creator Markiplier and the isolated vocal sound. The joke became popular because the clip is extremely short, instantly recognizable, and easy to place after a smash cut or zoom. It stayed in use as a general-purpose absurdist reaction sound, similar to how creators use quick punchline clips such as the Minecraft oof for instant comedic emphasis.