Place it at the exact cut where the reveal happens, then follow it with a shocked meme sound effect for the reaction beat. For a longer gag, pair it after the bass boost discord call so the two boosted hits escalate back to back.
About this sound
This is a two-second whistle styled after McDonald's, pushed with heavy bass until it rumbles and distorts. The sharp tone hits fast and the low end makes it feel overblown and disruptive. Listeners tend to hear it as an obnoxious, funny interruption that turns a plain moment absurdly dramatic.