Place this clip immediately after the repeated mistake, following a Windows XP Error Sound, then let a Womp Womp Fail Sound close the scene before the next cut and the next joke begins.
About this sound
This clip is a short Japanese anime voice line, just under four seconds long, in which a speaker drags out 'nandayo, mou, mata kayo' with rising, whiny frustration. It feels irritated and theatrical at the same time, turning ordinary annoyance into something funny and exaggerated. The stretched delivery makes the fed-up reaction easy to read instantly, even without subtitles.