Put it at the exact cut where the interruption happens, then follow it with Nope for a double dismissal. For a territorial version, open with What Are You Doing In My Swamp and place this clip as the reply that ends the exchange.
About this sound
This is a short Russian voice clip saying 'Ne meshai, ya zaniat' — 'don't disturb me, I'm busy' — delivered as a flat, impatient brush-off. It feels blunt and funny because the speaker sounds completely uninterested in being interrupted, which makes it a sharp way to shut down a distraction without starting an argument.