Place this clip at the reveal in a reaction edit, then follow it with the emotional damage meme sound for a second hit, or precede it with the bruh sound effect when the setup needs a deadpan lead-in before the refusal.
About this sound
A short voice clip of a drawn-out "oh hell no" reaction, stretched on the final word and lasting about three seconds. It gives listeners a mix of disbelief, disgust and instant refusal, like a hard no delivered for laughs. The clip is known from Vine, where it spread as a reaction sound in meme edits and compilations.