Place it at the exact frame of the reveal or correct answer, as the first reaction in the sequence. Follow it with the sexy sax sound if the scene keeps going, or end the sequence there for a clean, single-beat punchline in shorter edits.
About this sound
This is a brief voice clip of Mettaton saying Oh, yes in a slow, confident drawl, lasting just over one second. It feels smug, playful and deliberately over the top, like exaggerated approval. The clip comes from Undertale, the 2015 indie RPG by Toby Fox, where it matches the character's flamboyant, showman personality.