Place it as the payoff at the end of a short failed stunt, then follow it with a deadpan reaction for contrast. Pair it with another fart for a double hit, or let a goofy ahh sound set up the joke before it arrives.
About this sound
This clip is a single messy fart sound that sounds wrong in a funny way instead of like a normal toot. It lands as crude, silly and deliberately gross, the kind of noise that makes a room groan and laugh at the same time. It works as a quick prank noise, with no speech or music to explain it.