Place it at the end of a failed attempt, after the setup has finished, so it acts as the reaction. Follow it with the baby laughing meme for a quick mood switch, or pair it before the sad meow song to build a run of responses, then close with emotional damage for the final punch.
About this sound
This is a seven-second recording of a baby crying, a raw, wavering wail that rises and falls without words. It feels instantly distressing and a little awkward, the kind of sound that makes a room go quiet and everyone look round. Used on its own it reads as pure upset, but in a joke it turns that upset into exaggerated, helpless comedy.