This clip circulates as a user-uploaded reaction sound on meme soundboards, built entirely around the repetition of a single crude shouted line. It has no confirmed film, TV or game source and spread through short-form edits where creators needed an over-the-top verbal reaction to physical comedy and fails. The three-times structure is the hook, making it easy to sync to repeated hits or escalating reactions, and similar gross-out reaction clips like the damn sound effect are often used in the same style of edit.
About this sound
A crude shouted phrase repeated three times, used as a gross-out reaction sound for shocking, painful or humiliating moments in memes and edits.
This is a male voice loudly shouting the vulgar phrase "right up your poop chute" three times in quick succession, each repetition getting more emphatic. There is no music or other effects, just the raw, aggressive delivery of the line. The shock value and repetition make it land as a brutal punchline, like something just went horribly, painfully wrong for someone on screen. It works because it is crass, unexpected, and impossible to ignore once it plays.