The phrase is closely associated with Spider-Man, whose “spider-sense” warns him about danger in Marvel stories. Over time, it spread beyond comics and screen adaptations into internet memes, where creators use it for suspicion, intuition, and red-flag humor. Online, the sound often appears right before a reveal or a zoom-in, sometimes paired with a vine boom for extra emphasis. It stays popular because the meaning is instantly clear even in very short edits.