Place it right after a hopeful setup collapses in your edit, then follow it with sad violin to stretch the joke a beat longer before cutting to the next scene or a reaction shot.
About this sound
This is a short, downbeat SpongeBob meme clip built around a sad, drooping cartoon moment. It feels gently pathetic and funny at once, like a joke that has just run out of luck, and it works best as a small, sympathetic sigh rather than a big punchline. It comes from the world of that cartoon, where exaggerated sadness is instantly recognisable.