Put it at the end of a failed attempt, after the mistake is clear, then follow it with the SpongeBob Boowomp sound for a bigger cartoon letdown, or the Awkward Moment sound when the next shot is a reaction face.
About this sound
This is a short, roughly four-second cartoon reaction clip with a low, sinking tone and no spoken words, built for the instant after something goes wrong. It feels flat and awkward, turning a small failure into a joke instead of a drama. It comes from the SpongeBob SquarePants meme world, where the character's reactions are reused in short videos and soundboards.