Place it at the end of a failed attempt, just as the result lands, then follow it with SpongeBob Sad Song to stretch the defeat into a longer gag. For a SpongeBob-themed edit, open with You What SpongeBob Sound before the letdown so the crying becomes the payoff.
About this sound
This 5-second clip captures Squidward Tentacles sobbing in an over-the-top, cartoonishly miserable way, with soft, wailing cries that sound far bigger than the trouble behind them. It feels both sad and funny, turning disappointment into self-pity you can laugh at. It comes from SpongeBob SquarePants, where his grumpy personality makes the breakdown instantly recognisable.