Place it at the exact point of failure in a short edit, after the setup has made the mistake clear. Follow it with the Classic Error Sound for a second beat of failure, then cut to the SpongeBob Fail for the reaction shot that closes the joke.
About this sound
This clip is a pumped-up take on the familiar Windows error chime, turning a short system alert into a bigger, more dramatic fail signal. Its sharp, instantly recognisable tone makes a mistake feel sudden and comically serious, like a computer calling out the error for everyone to hear. It lands best as a quick burst of mock alarm.