This sound comes from Sweetie Belle, a character from My Little Pony: Friendship Is Magic, the animated television series that built a large online fan community in the 2010s. Short reaction lines from the show were widely clipped, shared and reused in fan videos, memes and edits because the voice acting is so expressive. This particular line remains useful because it communicates instant exasperation in just two seconds, making it easy to drop into reaction posts and quick comedic cuts.