Place it just before the reveal shot, then follow it with a Vine Boom for extra impact, or open with a Dramatic Dun Dun Dun before the laugh to build the reveal.
About this sound
This is a very short evil laugh, lasting about 1.3 seconds, delivered in a theatrical, exaggerated voice that sounds like a cartoon villain enjoying a secret plan. It feels smug, menacing and deliberately over the top, turning a simple moment into a dramatic reveal. It circulates under the Russian name Temnyi Prints Smekh, meaning Dark Prince laugh, as a meme reaction sound.