This sound comes from Team Fortress 2, Valve's class-based multiplayer shooter released in 2007. Short TF2 voice clips spread widely in gaming edits, YouTube memes, and reaction videos because they are expressive, instantly recognizable to players, and easy to time with a visual gag. The quick "No!" became especially useful as a compact reaction sound for failure, refusal, or disbelief. It still shows up in meme editing because classic TF2 audio has a distinct style that reads clearly even in very short clips.