This sound comes from Heavy, one of the main playable classes in Team Fortress 2, Valve's class-based shooter released in 2007. TF2 voicelines became popular far beyond the game because each class has a very distinct personality and delivery. Heavy clips in particular spread in meme edits, machinima, and short gaming videos thanks to his deep voice and exaggerated reactions. The crying line still gets used when creators want a recognizable game character to sell mock sadness, disappointment, or over-the-top emotional damage.