Place it at the peak of a scare, just after a slow reveal, then follow it with a Vine Boom and a Dun Dun Dun sting to close the scene on a final note.
About this sound
The clip is a very short, under-a-second scream in Tinky Winky's high, wavering voice, cut tight for a sudden shock. It feels startling and silly at once, more cartoon panic than real fear, which makes the scare land as a joke. The abrupt cut keeps the tension high without giving the listener time to settle.