El Macho is the main villain of Despicable Me 2 (2013), the masked luchador persona of Eduardo Pérez, a wealthy restaurant owner secretly plotting with the PX-41 serum, voiced by Benjamin Bratt. The character is famous for his theatrical Latin villain delivery, which made short quotes from him popular on meme soundboards. This 'Who's there?' line fits his suspicious, door-answering persona and circulates online as a reaction clip for knocks, entrances and surprise reveals in edits.