Place it immediately after the moment that earns the insult in your edit, with Anime Punch landing right after it to close the scene. If the blunder comes from a wrong answer, open with Wrong Answer Game Show first, then let this clip deliver the reaction.
About this sound
This is a roughly three-and-a-half-second Japanese shout, clipped and distorted until the voice cracks, yelling a drawn-out 'aaa' before calling someone trash and telling them to die. It feels loud, angry and deliberately over the top, like a rage reaction pushed past the point of being polite.