Place it at the exact point where the interruption happens, then follow it with Silence I Kill You to keep the other side quiet. For a back-and-forth argument, put No No Wait Wait just before it so the interruption builds first, then let this clip end the exchange.
About this sound
This is a short 1.7-second Dutch spoken line, 'Zwijg en laat mij uitspreken!', delivered as a firm demand to stop interrupting and let the speaker finish. The clipped, impatient delivery makes it feel blunt, exasperated and confrontational, like a verbal hand raised to cut off a talker mid-sentence.