Place this clip at the exact penalty moment in your edit, after the on-screen mistake lands and before the reaction shot. Follow it with the social credit siren for a bigger deduction gag, or lead in with a vine boom for a shorter reveal.
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The -250- clip is an 11-second sound effect built around a single penalty idea, presented as a stark minus score rather than a word or tune. It gives a listener a blunt, deflating feeling, like watching points vanish after a bad call, which makes the joke land as punishment rather than praise.