Place it directly on the impact cut, after a soft thud effect and before a short laugh track or surprised gasp from another character. It works best as the middle beat in a three sound chain, letting the hit land first and the reaction follow.
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This clip is a very short human voice shouting a sharp Ah of pain, lasting just 0.6 seconds with nothing else around it. It feels abruptly funny and a little dramatic, turning a tiny stub, bump or surprise hurt into an exaggerated reaction that listeners instantly read as comic suffering rather than real distress.