Place it right after the failed moment, then follow with the SpongeBob Fail sound for a second reaction, or open with the Dun Dun Dun sound to build suspense before the crowd reacts. Keep it as the punchline, not the setup, so the reaction closes the scene.
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This is a short crowd booing effect lasting about three and a half seconds, with overlapping voices jeering together as if reacting to a bad performance. It feels instantly mocking and dismissive, turning a failed moment into a shared joke. The sound lands as a playful put-down, sharp enough to sting without feeling hostile, and it works best when the target is clearly in on the gag.