Place this clip at the very end of a short edit, after the reaction shot lands. Follow a stunned Bruh Soundboard Button moment with this laugh for the payoff, or precede it with Emotional Damage Sound Button when the prank victim gets the worst of the exchange.
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This clip is a drawn-out evil laugh delivered as a loud MWAHAHA, lasting about four and a half seconds and credited to Bacon_Donut. It feels gleefully villainous and over the top rather than scary, like a cartoon bad guy celebrating a tiny win, which makes the laugh land as silly and smug instead of truly threatening.