Place it at the reveal in a horror edit, after a slow build and before the reaction shot. Start with Dun Dun Dun to set up the moment, then follow this clip with Vine Boom Sound Button for a heavier comic payoff.
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This is a seven-second spooky scare effect built to catch a listener off guard, with a sudden burst of eerie sound that feels like something jumping out in the dark. It gives a quick jolt of tension and nervous laughter, the kind of startle that works best when nobody expects it.