Place this clip at the peak moment of a reaction sequence, after a suspense beat and before a comedic deflation. Pair it with the sudden suspense sound first for tension, then follow it with Nelson's ha-ha for the payoff from the onlookers.
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This is a sub-second clip of Charlie letting out a single loud scream, with no other voice or music around it. The yell lands as startled and over-the-top, turning a small mishap into a moment of pure panic that listeners instantly read as a reaction rather than a real threat.