Place it at the end of the setup, just as the prank is revealed, then follow it with the Shocked Meme Sound for the reaction and the Oh No No No Laugh when the group realises it was a joke.
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The Chavezed clip runs for about 18 seconds and is presented as a prank sound, built for a quick comic interruption rather than a long listen. It gives a mischievous, caught-off-guard feeling that suits a joke landing at just the wrong moment for the person on the receiving end. Use it when you want the room to react before anyone has time to explain what just happened.