Place it at the very end of a failed attempt, after the setup has already shown what went wrong. Open with the Law and Order Dun Dun for a verdict, then close with this clip, or follow it with Mortal Kombat Fatality when the loss needs a bigger finish.
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The clip is three short spoken 'ded' sounds in a flat, clipped rhythm, one after another with no music or other voices. It lands as dry, deadpan mockery, giving a small defeat a blunt, official finish. Its repetition is what makes it funny: it states the end plainly, without drama, and leaves the silence after it to do the work.