Place it early in a horror edit, before the reveal, then follow it with the FNAF Jumpscare Scream for the payoff and the Fears to Fathom Notification Sound to reset the scene for the next scare.
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This is a just-over-ten-second horror-game cue centred on a feeling of dread, with tension that grows steadily while it plays. It feels uneasy and suspenseful, like standing in a dark hallway waiting for something unseen to move, and it puts the listener on edge before anything has happened. That slow, creeping unease is what makes it land.