Place it near the end of a prank reveal, after the trick is clear, then follow it with awkward crickets to let the joke settle before the next scene. For a lighter follow-up, use a chipmunk laugh in the next scene.
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This clip is a child's laugh lasting about six seconds, high-pitched and exaggerated, with a playful tone that makes the mischief sound deliberate. It feels creepy and funny at once, like a cartoon villain enjoying a secret plan, and the young voice makes the menace feel light rather than threatening.