Place it at the start of a hearing-test edit, then cut to the reactions before adding a laugh once someone responds, so the test, the response and the payoff stay in that order.
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A short 2.6-second high-pitched tone at 17.4 kHz, the kind of mosquito-like ringing used as a test tone and ringtone. It sounds like a thin, piercing whine that sits at the edge of hearing. It feels irritating and slightly tense for listeners who can hear it, and oddly silent for those who cannot, which makes the contrast itself the joke.