Put this clip just after the reveal frame, then follow the reaction with the dun dun dun sound for a staged build, or swap that second hit for a vine boom sound when the moment needs a heavier final beat in your sequence.
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This clip is a short vocal exclamation, a loud 'gah ey' blurted out over about four seconds with exaggerated, cartoonish energy. It gives a feeling of instant startled disbelief, like a reaction that is too big for the moment, which makes it funny rather than genuinely alarmed or tense.