Place it right at the moment a bad decision is made in your edit, then follow it with the Metal Gear Alert sound for the reaction, or switch to the Windows XP Error sound when the plan fails anyway.
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A very short voice clip of GLaDOS, the AI from Portal, flatly saying the single word stop. Her cold, synthetic delivery makes the command feel funny and slightly menacing at once, like a machine shutting down a human idea without any emotion or debate.