Place it in the middle of a scare sequence, after Enemy Spotted sets up the threat and before FNAF Jumpscare Scream delivers the payoff, with Inception Horn underneath the opening to build tension first.
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This four-second Gorgon clip is a single wavering vocal wail that spells itself out as weooooeoeoeoooeoeoeo, rising and falling without words. It feels eerie and unsettling, like something unseen is calling from just out of view, and it leaves a tense, creepy hush after it fades.