Put this clip as the reaction beat right after the main gag, then follow it with the Doge Bonk Sound Effect for a second hit, or precede it with No God Please No when the setup needs rising panic before the payoff.
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This clip is Patrick Star's stretched-out laugh lasting about three and a half seconds, a high, wobbling vocal burst with no words. It feels gleefully dim and contagious, turning embarrassment or confusion into pure silliness. It comes from Patrick in SpongeBob SquarePants, the Nickelodeon animated series.