Put it right after the Dun Dun Dun sting, once the serious moment is set, then cut to a Vine Boom hit so the edit ends on a heavy punch after the payoff.
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This short helium voice effect lasts just over three seconds and turns ordinary speech into a thin, high-pitched squeak that sounds tiny and cartoonish. It feels instantly silly and lighthearted, the kind of sound that makes a serious sentence collapse into laughter before it can finish.