Place this clip alone after the visual gag, with nothing competing underneath it, then follow the tail with a vine boom sound for a hard contrast, or swap to an anime wow sound if you want a lighter release instead.
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This clip is a sustained high-pitched voice effect that runs for about ten seconds, holding one thin, squeaky note in a stretched JPB delivery. It feels absurd and slightly tense, the kind of wail that turns a normal pause into a punchline because it keeps going well past where you expect it to stop.