The exact origin of this clip is not clearly documented, so it is best understood as an internet joke sound rather than a line from a known film or show. Its appeal comes from combining two instantly recognisable ideas, a cartoon honk and a pirate, into one short punchline that needs no explanation. Short meme sounds like this spread through edits, reaction videos and soundboards, where creators reuse the same few seconds again and again. That reuse is why it still works today, because viewers recognise the honk immediately and expect something ridiculous to follow.