Place it between the setup and the payoff, then follow it with a Bruh sound for the disbelief and an Emotional Damage sound for the consequence, so the edit moves from setup to reaction to fallout in that order.
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This is a short spoken meme line, lasting about five seconds, in a steady narrator voice built around the phrase 'it was at this moment that'. It gives the listener a tense, knowing pause, making the mistake that follows feel bigger and funnier and turning a small slip into a dramatic turning point.