Place it at the end of a reveal or just before a twist, then follow it with the Metal Gear Alert for a second payoff. For a longer villain sequence, pair it with an evil laugh and let Bowser go first so the second laugh closes the sequence.
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This is Bowser's short cackle from Super Mario 64, lasting just under three seconds, with a sharp, cartoonish villain voice that rises into a mocking laugh. It feels smug, mischievous and playfully menacing, like a boss who already knows he has won. The clip comes from the 1996 Nintendo 64 game, where players heard it during encounters with Bowser.